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. 2005 Jan 6;24(2):270–282. doi: 10.1038/sj.emboj.7600530

Figure 4.

Figure 4

DNA-dependent pelleting of Soj. Samples were performed in duplicates for reliability. Wild-type Soj is found exclusively in the supernatant when centrifuged in the presence of DNA but without ATP. In the presence of ATP, a substantial fraction (one-third) of wild-type Soj is found in the pellet. Hydrolysis-deficient Soj D44A is also found in the pellet in the presence of ATP and DNA, but to a greater extent than wild-type Soj (experiments performed at above saturating protein concentrations: two Soj dimers: one 24 bp binding site), indicating that ATP hydrolysis results in dissociation from the DNA. These results indicate that ATP-dependent dimerisation is a prerequisite for DNA binding.