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. 2016 Jun 30;8(19):30817–30829. doi: 10.18632/oncotarget.10339

Figure 3. Knockdown of SMAD4 or overexpression of SMAD4 had no effect on cell apoptosis, whereas it promotes or inhibits the cell cycle in NSCLC cells.

Figure 3

(A) and (D) Flow cytometry cell cycle analysis of A549 cells (silenced for SMAD4 or overexpressing SMAD4 and compared with NC or Vector controls). Cells were harvested at 72 h post-transfection and stained with propidium iodide. The percentages of cell cycle phases are shown in the insets of each panel, in which values represent mean ± SD of three measurements. (B) and (E) Flow cytometry apoptosis assay of A549 cells (silenced for SMAD4 or overexpressing SMAD4 and compared with NC or Vector controls). Cells were harvested at 72 h post-transfection and stained with Annexin V/FITC and propidium iodide (PI). (C) and (F) Expression of p21 (an inhibitor of cell proliferation) was analyzed by western blotting. Densitometry values for each protein were normalized to β-actin. *P < 0.05; **P < 0.01; ***P < 0.001.