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. 2016 Jun 30;8(19):30817–30829. doi: 10.18632/oncotarget.10339

Figure 6. Overexpression of an miR-205 mimic inhibits NSCLC cell viability and proliferation.

Figure 6

(A) QRT-PCR analysis of miR-205 levels in A549 cells transfected with miR-205 mimics or NC for 72 h. U6 was used as the internal control. SMAD4 mRNA expression in A549 cells transfected with miR-205 mimics or NC. β-actin was used as the internal control. (C) The SMAD4 protein levels in A549 cells transfected with miR-205 mimics or NC. (B) CCK-8 assay of cell viability in A549 cells transfected with miR-205 mimics or NC for 96 h. B Representative images of the clonogenic analysis for cell proliferation in A549 cells transfected with miR-205 mimics or NC. Bar charts showing clonogenic growth of A549 cells transfected with miR-205 mimics or NC. Values are represented as means ± SE from three measurements. **P < 0.01; ***P < 0.001.