Figure 5. Western blot in vitro experiment.
Ketamine administered during the second trimester down-regulated ERK, p-ERK, MSK, CREB, p-CREB, SYP, SYN, and PSD95 in PC12 cells during in vitro experiments. SCH772984 is the inhibitor of the ERK protein. Through the contrast between S group and C group, a very good effect of the inhibitor is revealed. (A) Representative immunoblots for expression levels of the corresponding proteins. (B–I) Histograms that quantify the corresponding proteins normalized to GAPDH, in which the data are expressed as mean percentages ± SEM of control mean values. *p < 0.05