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(A) CHIR-98014 and TWS119 rescued the ethanol-induced suppressive effect on p-GSK3β and β-catenin in BMSCs. The western blot shows p-Akt-Ser473, total-Akt, p-GSK3β, total-GSK3β. (B) Western blot of β-catenin after 72 hours. β-actin served as internal reference. (C) CHIR-98014 and TWS119 rescued the anti-osteogenic effect of ethanol in BMSCs, as shown by Alizarin red staining.
