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(A) Representative IHC staining with anti-CCR4 antibody for PRM, LNM and MBM specimens. Black bars indicate 100 μm. The insets show a magnification of the melanoma lesions. Black arrowheads indicate CCR4-positive melanoma cells. Yellow bars indicate 20 μm. (B) Box plot comparing H score for PRM, LNM and MBM. *P ≤ 0.05.
