Skip to main content
. 2004 Dec 23;24(2):418–427. doi: 10.1038/sj.emboj.7600534

Figure 1.

Figure 1

Targeted disruption of chicken FANCC loci in DT40 cells. (A) Schematic representation of partial chicken FANCC locus, the gene disruption construct, and the configuration of targeted allele. S, SacI; B, BamHI; H, HindIII. Only a single exon was deleted by the gene disruption. Arrowheads indicate the positions of primers used in RT–PCR. (B) Southern blot analysis of SacI-digested genomic DNA from cells with indicated genotypes using flanking probe as shown in (A). WT, wild type. (C) RT–PCR analysis of total RNA from wild-type and fancc cells. Primers were designed from FANCC sequences of upstream and downstream exons as shown in (A). As a control, the entire coding region of chicken Rad51 was amplified from each RT product. (D) Western blot analysis of whole-cell lysate prepared from wild-type and fancc cells probed with anti-chicken FANCD2 serum. Cells were treated with MMC (500 ng/ml) for 1 h, washed, and lysed 6 h later. L or S, long or short form of FANCD2, respectively.