Figure 7. PP1 treatment preserves expression of E-cadherin, ZO-1, claudin-1 and claudin-4 in cultured renal tubule epithelial cells exposed to oxidative stress.

(A) Cells incubated in DMEM-F12 medium containing 5% FBS were exposed to PP1 (5 μM) for 24 h in the presence or absence of 1MM H₂O₂. Cell lysates were subject to immunoblot analysis with antibodies to E-cadherin, ZO-1, claudin, claudin4, phospho-Src (Tyr416), and GAPDH. Representative immunoblots from three or more experiments are shown. Expression levels of indicated proteins (B–F) were quantified by densitometry and normalized with GAPDH. Data are represented as the mean ± SEM. Bars with different letters (A–C) are significantly different from one another (P < 0.05).