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. 2017 Mar 14;8(19):31297–31304. doi: 10.18632/oncotarget.16185

Figure 3. XN treatment alters autophagic influx.

Figure 3

(A) The HL-60 leukemia cells were treated with 15 μM XN for 48 h. The cells were then loaded with Cyto-ID for 30 min and immediately imaged by confocal microscopy. The cells were treated with 200 nM rapamycin overnight as positive control of autophagy. (B) The cells were treated with 5, 10, 15 μM XN for 48 h and immunoblotting was used to determine protein levels of Beclin- 1,LC3-II and p62. b-actin was used as a loading control. (C and D) The cells were treated with 3-MA (5 mM, 1h) and bafilomycin- A1 (20 nM, 1h) prior to treatment of XN at 15 μM for 48 h. The percentages of viable cells (C) and vacuolated cells (D) were measured. Data are expressed as mean ± S.D. from three independent experiments. **P < 0.01 vs. control group.