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AO can across the intact cell membrane in order to combined with DNA emits intense green fluorescence; EB can across the broken cell membrane in order to combined with DNA emits intense orange-red fluorescence. All the cells were count by the Imageproplus (IPP, version 6.0) for calculating the Cell survival rate (%). The Cell survival rate (%) = (normal cells / the total number of A549 cells) ×100%.
