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. 2017 Mar 18;8(19):31540–31553. doi: 10.18632/oncotarget.16361

Figure 3. HSP27 has a role in the maintenance of CSCs/CICs.

Figure 3

(A) Western blotting of HSP27 knockdown cells. HSP27 siRNA was transfected into HEC-1 cells. Twenty-four hours after transfection, total RNAs were purified and the expression of HSP27 was evaluated by Western blotting. β-actin was used as an internal control. (B) Aldefluor assay. ALDH activity of HSP27 knockdown cells was detected using the Aldefluor assay 48 hours after transfection. Percentage represents the proportion of ALDH1high cells. (C) Sphere formation assay. ALDH1high and ALDH1low cells derived from HSP27 SiRNA-transfected HEC1 cells were cultured in serum-free medium. After 2 weeks of culture in vitro, a picture of a tumor sphere was taken. The counts were examined for statistical significance using Student's t-test. Data represent means ± SD. *P values.