Table 2. Stem cell frequency of HSF1 mutant overexpressed HEC1 cells.

Cells	sphere-positive wells/total wells				CSC frequency	95% CI	†P value
	1 cell/well	10 cells /well	100 cells/well	1000 cells /well
S303A	0/48	2/48	21/48	48/48	1 in 175	121.3–252	0.64
S303E	0/48	3/48	16/48	48/48	1 in 212	148.3–304	0.78
S320A	0/48	7/48	18/48	48/48	1 in 168	116.3–242	0.524
S320E	0/48	4/48	21/48	48/48	1 in 163	112.7–235	0.45
S326A	2/48	4/48	10/48	48/48	1 in 246	174.2–349	0.382
S326E	3/48	5/48	25/48	48/48	1 in 118	82.9–167	0.039*
WT	0/48	6/48	27/48	46/48	1 in 173	119.7–249	0.576
Mock	0/48	5/48	20/48	47/48	1 in 197	137.4–284

^†Defference with Mock cells were caliculated by Chi-square test. *P < 0.05.