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. 2017 Mar 18;8(19):31601–31611. doi: 10.18632/oncotarget.16364

Figure 5. Effect of RIG-I like helicases knockdown on azithromycin (AZM)-augmented RV-induced expression of IFNβ and reduction of viral load in primary bronchial epithelial cells from asthmatic donors.

Figure 5

HBECs from asthmatic patients were transfected with siRNA specific for MDA5 or RIG-I and were then treated with azithromycin for 24 h before infection with 1MOI RV16 and continuous throughout the experiment. Cells were harvested for gene expression analysis 24 h post infection. Gene expression of IFNβ (A) and viral load (B) were measured by real-time PCR and data is presented as mean ± standard error of the mean (SEM) fold change of RV16 relative to UBC/GAPDH expression. Comparison of different groups was performed by Wilcoxon. #p < 0.05, ##p < 0.01, ###p < 0.001; *p < 0.05, **p < 0.01 vs. corresponding control. Data was obtained from 5 donors.