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. 2017 Mar 18;8(19):31626–31637. doi: 10.18632/oncotarget.16382

Figure 4. Melatonin protected BMSCs against FAC-induced intracellular ROS increase.

Figure 4

H2DCF-DA probe was applied to assessed the intracellular ROS production in FAC-induced BMSCs. ROS staining showed that exposed to FAC 200 μM for 24 h, BMSCs exhibited a gradual increase of intracellular ROS generation as illuminated by enhanced green staining in the cytoplasm. However, melatonin 100 μM suppressed the increase of ROS-positive cells with FAC treatment. Data are for three independent experiments (n = 3).