Table 2. Subgroup analyses of the associations between P16, RASSF1A, APC, and RARβ genes and NSCLC.
| Gene | Studies | Overall OR 95% CI | I2; P | P value | Cases | Controls |
|---|---|---|---|---|---|---|
| P16 | ||||||
| Method | ||||||
| MSP | 10 | 13.09 (5.77–29.71) | 29.0%; 0.178 | < 0.001 | 655 | 386 |
| nMSP | 2 | 67.21 ( 9.15–493.61) | 0.0%; 0.578 | < 0.001 | 185 | 165 |
| qMSP | 1 | 6.77 (1.87–24.51) | NA; NA | 0.004 | 63 | 36 |
| Race | ||||||
| Caucasians | 2 | 14.28 (6.93–29.42) | 0.0%; 0.965 | 0.022 | 146 | 29 |
| Asians | 11 | 15.92 (6.88–36.83) | 44.5%; 0.055 | < 0.001 | 757 | 558 |
| RASSF1A | ||||||
| Method | ||||||
| MSP | 7 | 22.17 (6.77–72.60) | 50.9%; 0.057 | < 0.001 | 473 | 300 |
| nMSP | 2 | 12.00 (1.12–129.03) | 61.8%; 0.105 | 0.04 | 159 | 68 |
| qMSP | 1 | 2.49 (0.29–21.57) | NA; NA | 0.409 | 76 | 30 |
| PCR | 1 | 76.83 (4.53–1302.49) | NA; NA | 0.003 | 62 | 46 |
| Race | ||||||
| Caucasians | 2 | 4.77 (0.83–27.50) | 2.8%; 0.310 | 0.08 | 166 | 44 |
| Asians | 9 | 21.15 (7.62–58.71) | 55.6%; 0.021 | < 0.001 | 604 | 400 |
| APC | ||||||
| Method | ||||||
| MSP | 3 | 10.32 (4.38–24.32) | 0.0%; 0.732 | < 0.001 | 260 | 134 |
| qMSP | 3 | 14.85 (0.68–322.24) | 81.4%; 0.005 | 0.086 | 241 | 134 |
| PCR | 1 | 99.10 (5.85–1679.50) | NA; NA | 0.001 | 62 | 46 |
| Race | ||||||
| Caucasians | 3 | 10.44 (0.62–174.92) | 77.7%; 0.011 | 0.103 | 256 | 94 |
| Asians | 4 | 17.17 (7.79–37.87) | 24.5%; 0.264 | < 0.001 | 307 | 220 |
| RARβ | ||||||
| Method | ||||||
| MSP | 4 | 10.02 (1.75–57.37) | 79.5%; 0.002 | 0.01 | 338 | 200 |
| nMSP | 2 | 13.65 (0. –805.76) | 86.8%; 0.006 | 0.209 | 183 | 156 |
| qMSP | 1 | 4.79 (1.28–17.93) | NA; NA | 0.02 | 70 | 80 |
| Race | ||||||
| Caucasians | 2 | 5.99 (1.97–18.27) | 0.0%; 0.526 | 0.002 | 162 | 94 |
| Asians | 5 | 7.94 (2.70–23.34) | 81.7%; < 0.001 | 0.003 | 429 | 342 |
Abbreviations: NA: not applicable; MSP: methylation specific PCR; PCR: polymerase chain reaction; qMSP; quantitative methylation specific PCR; nMSP: nested methylation specific PCR.