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. 2017 Mar 17;8(19):31876–31887. doi: 10.18632/oncotarget.16311

Figure 3. CCR7 detection, dynamics and activity on NKL3 cells.

Figure 3

A. NK cells labelled with FTC-anti-CCR7 antibodies, at 4°C and fixed with 4% PFA, capping does not occur. B. Patching upon warming the cells from 4°C to 25°C for 5 min. at 25°C. C. Warming for 15 mins, capping occurs. D. Quantification of the NKL3 cells adhesion to HPLNEC.B3 as number of NKL3 cells counted on the surface of HPLNEC.B3 cells monolayer (ten representative fields were counted), NKL3 were either maintained at room temperature or capping was induced (4-25°C) before and after preincubation with CCL21 *p<0.05, **p<0,01, N=3, # p<0.05, ## p<0,01 N=3.