(D) Comparison of cells with or without CD73 knockdown.
| Cell | No. of cells injected | No. of tumors/No. of mice | Mean latency, days (95% CI) | Maximum tumor volume, mm3 | Day of tumor harvest |
|---|---|---|---|---|---|
| Control shRNA | 5 × 104 | 4/8 | 48.6 ± 5.1 | 1175 | 110 |
| 5 × 103 | 3/8 | 63.0 ± 5.2 | 782 | 110 | |
| 5 × 102 | 1/8 | 60 | 8 | 110 | |
| CD73 shRNA | 5 × 104 | 3/11 | 53.3 ± 5.8 | 742 | 110 |
| 5 × 103 | 1/11 | 62 | 329 | 110 | |
| 5 × 102 | 0/11 | ‡ | - | 110 |
*Nonobese diabetic/severe combined immunodeficient (NOD-SCID) mice were used for A-C and nude mice for D. Tumor cells, as indicated, were injected in a 200 μL mixture with matrigel bilaterally into mouse inguen (one tumor per site). Sorted cells were injected within 1–2 hours after sorting. The mean latency was calculated as the time from tumor cell injection to appearance of measurable tumors (at least 5 mm × 5 mm). The 95% confidence interval (CI) was calculated for each tumor group with more than one tumor. Day of tumor harvest was determined from the day of cell injection to the determined day or when mice appeared with the syndrome of impending death. ‡ No palpable tumors were present at the day of tumor harvest.