Figure 2. SERT is expressed in breast tumor cell lines representative of all the molecular subtypes of breast cancer.

Western blot performed with breast tumor cell line lysates (35 μg) and mouse brain tissue after electrophoresis in 5-15% gradient polyacrylamide gels reveal that the rabbit polyclonal antibody identifies SERT species that migrate between molecular mass markers of 63 kDa and 75 kDa, and between 48 kDa and 63 kDa. The arrowheads identify the glycosylated and non-glycosylated SERT species. Non-glycosylated SERT co-migrated with α-tubulin, the loading control, which is shown in the bottom-most panel.