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. 2017 Apr 17;8(19):32157–32170. doi: 10.18632/oncotarget.17150

Figure 4. IE86 interacts with endogenous ATF5 but not with dnATF5.

Figure 4

(A) U87 cells were transfected with or without IE86 constructs. Immunoblotting analyses of immunoprecipitates using indicated antibodies were performed. (B) C6 glioma cells were transfected with IE86 constructs and transiently transfected with a vector expressing HA-ATF5 or one expressing dnATF5. Expression of IE86 and FLAG-HA-ATF5 were detected using antibodies against IE86 and HA as indicated. (C) Immunofluorescence staining of ATF5 (red) and IE86 (green) in U87 cells U87 cells were transfected with IE86 constructs, fixed at 48 h posttransfection, stained with antibodies, and visualized using an Olympus confocal microscope system. The images of IE86 (green), ATF5 (red), and the morphology were used to generate the composite images. The colocalization of the two proteins is in the nucleolus. Bar scale is 10 μm.