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. 2017 Apr 17;8(19):32157–32170. doi: 10.18632/oncotarget.17150

Figure 5. IE86 acetylates ATF5 at K29 through p300.

Figure 5

(A) IE86 overexpression enhances acetylation of ATF5.U87 cells were transiently transfected with or without IE86 constructs and followed with or without serum deprived and TSA treatment. Anti-ATF5 immunoprecipitates from cells were immunoblotted with indicated antibodies. (B) ATF5 acetylation induced by IE86 is p300-dependent. U87 cells were transfected with IE86 constructs and with or without shRNA against p300. Anti-ATF5 immunoprecipitates from cells were immunoblotted with indicated antibodies.