LacZ staining of E8.5 Flk-1+/KO, Flk-1KO/1173F, and Flk-1KO/KO embryos. (A–C) Whole-mount LacZ staining. (D–R) Transverse sections of LacZ-stained embryos. (D–F) Head region. (G–I) Heart region. (J–L) Somatic region. (M–O) Tail region. (P–R) Yolk sac. al, allantois; bi, blood island; da, dorsal aorta; ec, endocardium; g, gut; va, vitelline artery; ys, yolk sac. (Scale bars: A–C, 500 μm; D–R, 100 μm.)