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. 2017 May 12;198(12):4792–4801. doi: 10.4049/jimmunol.1502532

FIGURE 4.

FIGURE 4.

MerTK inhibitor UNC569 blocks LPS-induced phagocytosis. (A) BV-2 cells were treated or not with UNC569 (500 nM) for 30 min and then were treated or not with 100 ng/ml LPS for 24 h. Neuronal debris was added for 2 h, and the amount of debris that was phagocytosed was quantified. Absolute phagocytosis of the vehicle control cells: 6.57 ± 2.47% (n = 3). (B) Alternatively, live PC12 cells were added for 4 h instead of debris, and the amount of BV-2 phagocytosis of PC12 cells was quantified. Absolute phagocytosis of the vehicle control cells: 10.68 ± 0.98% (n = 5). (C) Primary glial cultures were treated or not with UNC569 (500 nM) for 30 min and then were treated or not with 100 ng/ml LPS for 24 h, neuronal debris was added for 2 h, and the amount of debris that was phagocytosed was quantified. Absolute phagocytosis of the vehicle control cells: 11.92 ± 2.70% (n = 3). (D) TNF-α levels in culture media were measured in the same experiment (mean ± SEM; n = 4). *p < 0.05, ***p < 0.001 versus vehicle control in the absence of LPS. #p < 0.05, ###p < 0.001 LPS versus LPS + UNC569. All p values were calculated using the Tukey post hoc test.