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. 2017 May 12;198(12):4792–4801. doi: 10.4049/jimmunol.1502532

FIGURE 5.

FIGURE 5.

LPS upregulates MerTK expression and Gal-3 binding to BV-2 cells. (A) MerTK expression and Gal-3 binding were increased in response to LPS stimulation. Note that Gal-3 can bind cells that express MerTK. Scale bar, 50 μm. (B) Representative coimmunoprecipitation. A total of 250 μg of cellular lysates was immunoprecipitated with MerTK Ab or control IgG, and then Western blotted with MerTK and Gal-3 Abs. (C) Quantification of coimmunoprecipitation. MerTK and Gal-3 physically interact, and this interaction was significantly increased by LPS stimulation. Black bars, vehicle treatment; white bars, LPS treatment. *p < 0.05 versus vehicle (absence of LPS, immunoprecipitated with MerTK Ab). n = 3. (D) Gal-3 binding to immunoprecipitated MerTK. Cellular lysates were immunoprecipitated with MerTK Ab (C terminus) or control IgG and then immunoprecipitated beads were incubated with TAMRA-stained rGal-3 at 37°C for 1 h. Mean FL3 of beads were measured. *p < 0.05 versus corresponding control IgG group. #p < 0.05 versus vehicle (absence of LPS, immunoprecipitated with MerTK Ab). n = 3 independent experiments each.