Table 2.
Number | Primers | Sequence (5′–3′) | TAP | NPB | PPB (%) |
---|---|---|---|---|---|
1 | UBC857 | AGAGAGAGAGAGAGAGYG | 17 | 12 | 70.59 |
2 | UBC818 | CACACACACACACACAG | 7 | 2 | 28.57 |
3 | UBC850 | GTGTGTGTGTGTGTGTYC | 9 | 4 | 44.44 |
4 | UBC840 | GAGAGAGAGAGAGAGAYT | 10 | 2 | 20.00 |
5 | UBC812 | GAGAGAGAGAGAGAGAA | 7 | 1 | 14.29 |
6 | ISSR04 | GAGAGAGAGAGAGAGACT | 10 | 2 | 20.00 |
7 | ISSR11 | ACACACACACACACACTG | 11 | 8 | 72.73 |
8 | ISSR21 | GAGAGAGAGAGAGAGATG | 7 | 2 | 28.57 |
9 | ISSR25 | ACACACACACACACACCT | 10 | 4 | 40.00 |
10 | ISSR24 | ACACACACACACACACCA | 13 | 7 | 53.85 |
11 | ISSR36 | GCGTCTCTCTCTCTCTC | 8 | 2 | 25.00 |
12 | ISSR47 | AGAAGAAGAAGAAGAAGAAGA | 7 | 4 | 57.14 |
13 | ISSR02 | AGAGAGAGAGAGAGAGCC | 12 | 10 | 83.33 |
14 | ISSR05 | GAGAGAGAGAGAGAGACC | 7 | 5 | 71.43 |
15 | ISSR06 | GAGAGAGAGAGAGAGACG | 12 | 9 | 75.00 |
16 | ISSR08 | GTGTGTGTGTGTGTGTCC | 15 | 14 | 93.33 |
17 | ISSR12 | CCCTCCCTCCCTCCCT | 3 | 1 | 33.33 |
18 | ISSR14 | CTTCACTTCACTTCA | 7 | 3 | 42.86 |
19 | ISSR10 | ACACACACACACACACTA | 4 | 1 | 25.00 |
20 | ISSR15 | GGAGAGGAGAGGAGA | 7 | 1 | 14.29 |
| |||||
Total | 183 | 94 | 913.75 | ||
| |||||
Mean | 9.15 | 4.7 | 45.69 |
TAP, total amplified products; NPB, number of polymorphic bands; PPB, percentage of polymorphic bands.