Table 1. Macromolecule-production information.

Source organism	L. rhamnosus GG (ATCC 53103)
DNA source	L. rhamnosus GG (ATCC 53103)
Forward primer†	5′-CCACATTGGGTTCAGAATTCTGATCAAACTG
Reverse primer‡	5′-TGCGCCAATCGGACTCGAGCGGCAAATAAC
Cloning vector	pET-28b(+) (Novagen)
Expression vector	pET-28b(+) (Novagen)
Expression host	E. coli BL21 (DE3) pLysS
Complete amino-acid sequence of the construct produced§	MGRDPNSDQTAEIVIHKRIYRDIRQPEDVWYENDGHRIDPNNPDKDGYKLLSKTSGLNGANFEVYDASSLLKPNMTPEAIRALVDRYQNMTRKQALKFARANLKLAGQGNKGIGLMNTKNDPTLGEDGISRITVSVDQQAPTKAYLMIEVAPDPSTELNVDLERKSSPMLVVFPVTDPISGNPLQTIHLYPKNVGYVRDPYFFKFGVHPDGTSKRLAGAIFAIYRIENGKKLYLDMSPVTDLRNKWVSTTDPLHDDRVNKFVSDQDGLVNTGERFLPAGEYFFEELQGVPGYEVDAKSRAIKIEIPDSWEDEDGNRRFVLIDGQPMQENFGGVVTPEMISSGYPRVYNYADKQASTTGDQTAGPSTTQLGNHGQDTNGTGTRTPKRQSGYLPLEHHHHHH

^†The EcoRI site is underlined.

^‡The XhoI site is underlined.

^§The cloning artifacts are underlined.