Table 2. Crystallization.

	Trigonal form (native)	Orthorhombic form (native)	Orthorhombic form (SeMet derivative)
Method	Hanging-drop vapour diffusion	Hanging-drop vapour diffusion	Hanging-drop vapour diffusion
Plate type	VDX plate (Hampton Research)	VDX plate (Hampton Research)	VDX plate (Hampton Research)
Temperature (K)	295	295	295
Protein concentration (mg ml−1)	100	100	100
Buffer composition of protein solution	50 mM Tris–HCl pH 8.0, 150 mM NaCl, 0.1 M bis-tris propane pH 8.5, 0.3 M sodium formate, 22% PEG 3350	50 mM Tris–HCl pH 8.0, 150 mM NaCl, 0.1 M bis-tris propane pH 8.5, 0.25 M sodium formate, 25% PEG 3350, 0.2 M sodium iodide, 0.02 M L-proline	50 mM Tris–HCl pH 8.0, 150 mM NaCl, 0.1 M bis-tris propane pH 8.5, 0.3 M sodium formate, 30% PEG 3350, 1 M sodium iodide, 0.1 M L-proline
Volume and ratio of drop	2 µl, 1:1	2 µl, 1:1	2 µl, 1:1
Volume of reservoir (ml)	1	1	1