Figure 4.

Pathway of intracellular trafficking of Aβ‐42 and its localization in ER. Representative confocal microscopic images of Jurkat T cells both control and with added 25‐OH (5 and 10 μm) after exposure to protofibrillar Aβ‐42. Observation of microtubules were performed in its polymerized and depolymerized microtubule state. Green and red fluorescence represents Oregon green (488 nm) and HiLyte Fluor‐labeled Aβ‐42 (555 nm), respectively. Observation of Aβ‐42 in the ER was observed in the provided images. Green and blue fluorescence represents HiLyte Fluor‐labeled Aβ‐42 (488 nm) and ER (350–460 nm), respectively. Images of one representative experiment out of three performed. Number of cells observed were 150–200. Scale bars = 10 μm.