Table 3.
Comparisons of the expressions of genes located on the TLR‐cytokine axis using 2−ΔΔCT method. All analyses were performed in triplicate. MSE: mean squared error
| Genes | Group BT | Group CO | MSE | p value |
|---|---|---|---|---|
| TLR1 | 0.95 | 1.01 | 0.07 | .733 |
| TLR2 | 1.62 | 1.00 | 0.04 | .009 |
| TLR4 | 0.92 | 1.01 | 0.06 | .055 |
| TLR5 | 1.55 | 1.00 | 0.04 | .025 |
| TLR6 | 0.95 | 1.00 | 0.04 | .538 |
| TLR10 | 1.02 | 1.00 | 0.04 | .819 |
| MyD88 | 1.49 | 1.01 | 0.08 | .011 |
| IL‐1ß | 0.79 | 1.01 | 0.04 | .015 |
| IL‐6 | 1.10 | 1.00 | 0.04 | 0.167 |
| IL‐10 | 1.07 | 1.01 | 0.06 | .527 |
| TNF‐α | 0.95 | 1.01 | 0.07 | .176 |
| IFN‐γ | 0.60 | 1.01 | 0.05 | .039 |
GAPDH (glyceraldehyde 3 phosphate dehydrogenase) was used as the housekeeping gene.
TLR = toll‐like receptor; MyD88 = myeloid differentiation primary response 88; IFN‐γ = interferon‐gamma; IL = interleukin; TNF‐α = tumor necrosis factor alpha.