Fig. 1.

DC depletion results in diminished reactivity of splenic NK T cells, whereas Kupffer cell depletion results in diminished reactivity of liver NK T cells to α-GalCer. (A) Groups of three C57BL/6 Tg mice were injected with DT or PBS and, 24 h later, were treated with 1 μg of α-GalCer. Two hours after glycolipid treatment, splenocytes and hepatic lymphocytes were isolated and subjected to ICCS. NK1.1+ and CD3+ NK T cells were gated from both cell populations. (B) Groups of three WT C57BL/6 mice were injected with either clodronate-loaded liposomes (CL2) or PBS-loaded liposomes (PBS) and, 48 h later, were treated with 1 μg of α-GalCer. Two hours after glycolipid treatment, splenocytes and hepatic lymphocytes were isolated and subjected to ICCS. NK1.1+ and CD3+ NK T cells were gated from both cell populations. The data shown come from one of three independent experiments with similar results.