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(a) Fusobacterium nucleatum radD cmpA double mutant (strain BL83) was constructed by insertion of the inactivation plasmid pBPL9 into cmpA (Fn1554) in a radD::catP mutant background. Black arrows indicate the location of primers used for mutant construction and analysis. (b) Confirmation of plasmid insertion into cmpA by PCR analysis of the cmpA mutant (m) with wild‐type (WT) as the control
