Table 1.

Bacterial strains and plasmids used in this study

Species	Strains	Description	Source
Escherichia coli	DH10B™	F‐ mcrA Δ(mrr‐hsdRMS‐mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG	Thermo Fisher
Fusobacterium nucleatum	ATCC 23726	ssp. nucleatum wild type	ATCC
	ΔFn0254	ATCC 23726 Fn0254::pIP0254	Kaplan et al. (2009)
	ΔFn1526 (radD)	ATCC 23726 Fn1526::pIP1526	Kaplan et al. (2009)
	ΔFn1554 (cmpA)	ATCC 23726 Fn1554::pIP1554	Kaplan et al. (2009)
	ΔFn1893	ATCC 23726 Fn1893::pIP1983	Kaplan et al. (2009)
	ΔFn2047	ATCC 23726 Fn2047::pIP2047	Kaplan et al. (2009)
	aim1	ATCC 23726 aim1::pIPaim1	Kaplan et al. (2009)
	BL83	ATCC 23726 radD::catP fn1554::Erm	This study
Streptococcus gordonii	ATCC 10558	S. gordonii wild type	ATCC
	ATCC 51656	S. gordonii wild type	ATCC
	DL1	S. gordonii wild type	ATCC
	V288	S. gordonii wild type	ATCC
	BL98	V288 attB::mCherry	This study
Streptococcus sanguinis	ATCC 10556	WT S. sanguinis	ATCC
Porphyromonas gingivalis	4612	WT P. gingivalis	Lamont et al. (1995)

Plasmid	Description	Purpose	Source
pHS70	F. nucleatum suicide vector; ermB	Suicide plasmid	S. Haake (unpublished data)
pBPL9	pHS31 with Fn1554_ inserted into SnaBI site	Gene inactivation plasmid	This study
pVA8912:mCherry	pVA8912 carrying mCherry under the control of ldh promoter.		Vickerman et al. (2015)