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EM images of negatively stained fields of 20S proteasome complexes showing the effect of altering the surface charge of the carbon support film on the orientation of the human 20S proteasome. (a) Glow discharge of the EM grid in a partial vacuum of atmospheric air results in a strongly biased proteasome orientation with predominant top views. (b) Glow discharge in a partial vacuum containing pentylamine vapour results in a predominance of side views.
