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. 2005 Jan 14;102(4):1217–1221. doi: 10.1073/pnas.0409252102

Fig. 2.

Fig. 2.

Xenopus melanophores were seeded in 96-well plates and kept in the dark for a minimum of 3 days. (A) cGMP determination in Xenopus melanophores in the dark and after a 1-min light exposure, in the absence or presence of the guanylyl cyclase activator, YC-1, at 2 × 10–7 M. Each bar is the mean ± SE (n = 6) of femtomoles per 104 cells. Asterisks indicate a significant difference from the dark control. (B) Melanosome dispersion in response to 10–4 M 8-bromo-cGMP in the dark (•), as compared with the light-evoked response (○). (C) Light-evoked melanosome dispersion in Xenopus melanophore in the absence (○) or presence (▪) of the PKG inhibitor, DT-2, at 10–5 M. In B and C the cells were pretreated with 10–9 M melatonin for 90 min, and the absorbance readings were expressed as a percentage of the absorbance of cells exposed to the EI50 for 60 min (considered as 100%). Each point is the mean ± SE (n = 6) at the times noted. Asterisks indicate a significant difference from the light response.