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. 2017 May 25;8:15201. doi: 10.1038/ncomms15201

Figure 3. miR-29b is sufficient to induce muscle atrophy in vitro.

Figure 3

(a) qRT–PCR analysis showed increased miR-29b, but not miR-29a or miR-29c expressions, in C2C12 myotubes transfected with miR-29b mimic compared to negative control (NC mimic) (n=6 per group). (b) Immunofluorescent staining for myotubes showed decreased myotube diameter in C2C12 myotubes transfected with miR-29b mimic (n=4 per group, scale bar, 100 μm). (c) qRT–PCR analysis showed upregulated Atrogin-1 and Murf-1, but downregulated MHC expressions in C2C12 myotubes transfected with miR-29b mimic (n=6 per group). (d) qRT–PCR analysis showed increased autophagy-related gene expressions in C2C12 myotubes transfected with miR-29b mimic (n=6 per group). (e) qRT–PCR analysis showed upregulation of other ubiquitin ligases-related gene expressions in C2C12 myotubes transfected with miR-29b mimic (n=6 per group). (f) qRT–PCR analysis showed increased miR-29b expression in myotubes differentiated from primary myoblasts transfected with miR-29b mimic (n=6 per group). (g) qRT–PCR analysis showed increased Atrogin-1 and Murf-1 expressions when myotubes differentiated from primary myoblasts were transfected with miR-29b mimic (n=6 per group). (h) Immunofluorescent staining for myotubes differentiated from primary myoblasts showed that myotube diameter was decreased after transfected with miR-29b mimic (n=4 per group, scale bar, 100 μm). Error bars, s.e.m. An unpaired, two-tailed Student's t-test was used for comparisons between two groups. *P<0.05, **P<0.01.