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. 2017 May 25;8:15201. doi: 10.1038/ncomms15201

Figure 5. IGF-1 and PI3K(p85α) are identified as two target genes of miR-29b.

Figure 5

(a) TargetScan and Luciferase reporter assay showed that IGF-1 and PI3K(p85α) were two direct targets of miR-29b (n=5 per group). (b) Western blot showed that miR-29b negatively regulated IGF-1 and PI3K(p85α) in C2C12 myotubes (n=3 per group). (c) Western blot analysis for the AKT/FOXO3A/mTOR pathway (AKT, FOXO3A, mTOR, P70S6K, 4EBP1) showed decreased phosphorylation levels of AKT (S473), FOXO3A (S253), mTOR and P70S6K in C2C12 myotubes transfected with miR-29b mimic (n=3 per group). (d) Western blot analysis for the AKT/FOXO3A/mTOR pathway (AKT, FOXO3A, mTOR, P70S6K, 4EBP1) showed increased phosphorylation levels of AKT (S473), FOXO3A (S253), mTOR and P70S6K in C2C12 myotubes transfected with miR-29b inhibitor (n=3 per group). Error bars, s.e.m. The presented blots are representative samples of three independent experiments. An unpaired, two-tailed Student's t-test was used for comparisons between two groups (b–d). One-way ANOVA test was performed to compare multiple groups followed by Bonferroni's post hoc test (a). *P<0.05, **P<0.01.