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. 2017 May 25;8:15201. doi: 10.1038/ncomms15201

Figure 7. YY1 negatively regulates miR-29b in muscle atrophy.

Figure 7

(a) qRT–PCR analysis showed downregulated IGF-1 but not miR-29b expression in C2C12 myotubes transfected with IGF-1 siRNAs compared to negative control (NC siRNA) (n=6 per group). (b) qRT–PCR analysis showed down-regulated Yy1 while upregulated miR-29b expressions in C2C12 myotubes transfected with Yy1 siRNAs compared to negative control (NC siRNA) (n=6 per group). (c) qRT–PCR analysis showed decreased Yy1 mRNA level in the gastrocnemius from Den-, Dex- and fasting-induced muscle atrophy models (n=5 per group). (d) Western blot analysis showed decreased YY1 protein level in the gastrocnemius from Den-, Dex- and fasting-induced muscle atrophy models (n=3 per group). (e) Immunofluorescent staining for C2C12 myotubes showed decreased myotube diameter when transfected with Yy1 siRNAs (n=4 per group, scale bar, 100 μm). (f) qRT–PCR analysis showed increased Atrogin-1 and Murf-1 expressions when C2C12 myotubes were transfected with Yy1 siRNAs (n=6 per group). Den, Denervation; Dex, dexamethasone. Error bars, s.e.m. The presented blots are representative samples of three independent experiments. An unpaired, two-tailed Student's t-test was used for comparisons between two groups (c,d). One-way ANOVA test was performed to compare multiple groups followed by Bonferroni's post hoc test (a,b,e,f). *P<0.05, **P<0.01.