. Author manuscript; available in PMC: 2017 Jun 5.

Published in final edited form as: Enzymes. 2016 Mar 28;39:293–323. doi: 10.1016/bs.enz.2016.02.002

Table 1. Kinetic Analysis Of Pv Rdrp, Hiv-1 Rt, Rb69 Dddp, And T7 Ddrp Supports General Acid Catalysis In Nucleotidyl Transfer [37].

Parameter Measured	PV RdRp		HIV RT		RB69 DdDp		T7 DdRp
	Lys-359	Leu-359	Lys-220	Leu-220	Lys-560	Leu-560	Lys-631	Leu-631
	k_pol (s⁻¹)	50 ± 5	1 ± 0.1	60 ± 5	0.3 ± 0.1	200 ± 10	0.10 ± 0.01	60 ± 5	0.6 ± 0.1
K_d,app (μM)^a	200 ± 20	700 ± 80	7 ± 1	5 ± 2	40 ± 5	1000 ± 100	300 ± 30	5.0 ± 1.0 × 10⁴^b
SDKIE^c	3.0 ± 0.3	2.5 ± 0.3	2.2 ± 0.4	1.8 ± 0.4	4.2 ± 0.2	1.8 ± 0.2	5.2 ± 0.5	2.6 ± 0.5^b
PI^d	2	1	2	1	2	1	2	nd^e

K_d,app is for (d)ATP.

K_d,app, k_pol, and SDKIE values listed for T7 K631L were obtained by using data collected with 80 mM ATP, a subsaturating concentration.

SDKIE is solvent deuterium kinetic isotope effect, calculated as k_obs in H₂O/k_obs in D₂O at saturating [(d)ATP].

PI is proton inventory, calculated from a plot of k_n/k₀ as a function of n. The data were fit to a modified Gross–Butler equation for either a two-proton-transfer model or a one-proton-transfer model. The value reported is the proton-transfer model that best fits the data.

Not determined.