Fig. 10.

Comparison of siglec ligands and mucins in human airway and lung tissue extracts and human airway cells. Equivalent aliquots of extracts of cultured human airway cells (TEC and TGC), secretions from cultured intact bronchus (BrE), detergent extracts of  LP and sequential detergent and GuHCl extracts of trachea (TrD, TrG) were resolved on replicate composite agarose-acrylamide gels, blotted to PVDF membranes, and replicate blots probed with precomplexed Siglec-8-Fc or Siglec-9-Fc or with antibodies to the indicated human mucins followed by appropriate HRP-conjugated secondary antibodies prior to enhanced chemiluminescent detection. Migration positions of very high molecular weight markers purified from rat soleus muscle are shown on each side of the panels.