Figure 4. Dynamic control of gene expression for increasing docosanol production.

(a) Schematic pathway describing the reason for growth defect. (b) Schematic illustration describing the dynamic control system, where cell growth and docosanol production are separated into two different phases using carbon source dependent promoters. (c) Docosanol production and final culture OD₆₀₀ in relevant strains. Statistical analysis was performed using a Student’s t-test (one-tailed; *P<0.05, **P<0.01 and ***P<0.001; two-sample unequal variance). At least two independent measurements were performed for each experiment and the mean±s.d. of three biological replicates of a representative measurement is shown. All cells were grown and induced as described under experimental procedures.