| Diluted lysate (from Step 5) | 5 μL |
| Forward primer (20 μM) | 1 μL |
| Reverse primer (20 μM) | 1 μL |
| dNTPs (1 mM) | 5 μL |
| 10× PCR buffer | 5 μL |
| Taq (or other thermostable) DNA polymerase | 2 units |
| Water | to 50 μL |
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