| pPC86 plasmid | 10 μg |
| Bovine serum albumin (1 mg/mL) | 4 μL |
| 10× Restriction buffer | 4 μL |
| SalI | 2 μL (20 units) |
| BglII | 2 μL (20 units) |
| Water (molecular biology grade) | to 40 μL |
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