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. Author manuscript; available in PMC: 2018 May 15.
Published in final edited form as: J Immunol. 2017 Apr 19;198(10):3939–3948. doi: 10.4049/jimmunol.1601078

Figure 1. Proteomic workflow for discovery and validation of novel T-bet phosphorylation sites.

Figure 1

A, Mouse T-bet protein sequence was used as an input for phosphorylation site prediction analysis using NetPhos program. T-bet phosphorylations with the highest predicted scores were chosen for LC-MRM-MS method development. B, Phosphorylation sites and their NetPhos scores that were chosen for preliminary MRM method development.