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. Author manuscript; available in PMC: 2017 Dec 20.
Published in final edited form as: Biochemistry. 2016 Dec 8;55(50):7014–7022. doi: 10.1021/acs.biochem.6b00914

Figure 4.

Figure 4

Non-pseudo-first-order conditions. The free ligand concentration was tracked during simulations, and relaxation curves were fit with single exponentials to estimate an observed rate for non-pseudo-first-order conditions. (A) Observed rate as a function of total ligand concentration with different concentrations of binding partner, A (shown in the legend). (B) Observed rate as a function of total A concentration in the presence of different concentrations of total internal ligand, L (shown in the legend).