Table 1. Stability of selenium-binding proteins.
| Remaining amount, %
|
Binding ratio (Se:protein)
|
||
|---|---|---|---|
| Protein | Selenium | Protein | |
| Rodanese | 30.9 | 67.3 | 0.5 |
| 3-MST | 31.9 | 94.8 | 0.3 |
| GAPDH | 56.6 | 76.1 | 1.4 |
Each flow-through fraction (0.5 ml) from the FPLC column was stored in a 1.5-ml sample tube under aerobic conditions at 4°C for 4 weeks. Concentrations of selenium and protein in soluble supernatant fractions obtained after centrifugation are represented as the percentage of the corresponding values obtained before sample storage.