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. 2017 May 26;8:15605. doi: 10.1038/ncomms15605

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Copyright © 2017, The Author(s)

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(a) An homozygote M82 CRISPR mutant (+A, +A) expressing 35S:Cas9 and u6-26:Ps#2-sgRNA was crossed with S. pimpinellifolium^LA1578. The F₁ is expected to give red fruits without DNA DSB and yellow fruit in case that the break was repaired by NHEJ, non-crossover or crossover. The SNPs pattern is allowing differentiating between repair mechanisms. Triangles are for SNPs; lightning mark the DSB site; blue line is for NHEJ indels. (b) Analysis of DNA DSB flanking markers in F₂ and F₃ plants. Red- homozygote for S. pimpinellifolium^LA1578 SNPs; yellow- homozygote for M82 SNPs (including the +A CRISPR-Cas9 mutant); orange- heterozygote; empty cells are for missing data; lightning- DSB site.