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Abi-1 is required for WAVE2 membrane translocation. B16F1 cells were transfected with pEGFP-Abi-1 and either DsRed WAVE2 (a and b) or DsRed WAVE2ΔWHD (d and e) or with pEGFP-Abi-1ΔCoil and DsRed WAVE2 (c) and the subcellular distribution of these proteins as well as Alexa 350-phalloidin-stained F-actin examined in resting (–) or 6-h FN-stimulated (+) cells by immunofluorescent microscopy. Images are representative of six independent experiments.
