Figure 3 .
Western blot analysis of the recombinant NS4B protein with mouse anti-HIS antibodies. Lane M: Protein marker; lane 1: purified NS4B (44 kDa); lane 2: fraction from wash step of Ni-NTApurification; lane 3: total protein of E. coli BL21carrying pET102-NS3 without IPTG induction; lane 4: Total protein of Escherichia coli BL21 as negative control