(A) Fusion reactions were performed using wild type or dgk1Δ vacuoles. Individually, reactions were treated with PS buffer (Control), or antibodies against Nyv1 (17 μg/ml), Vam3 (27 μg/ml), Vps33 (5 μg/ml) or Ypt7 (8 μg/ml). (B–D) Dose response curves of anti-Ypt7 IgG (B), Gyp1-46 (C), and GDI (D). Reactions containing wild type of dgk1Δ vacuoles were incubated for 90 min at 27°C and tested for fusion. Error bars represent S.E.M. (n=3). *P<0.05 (one-way ANOVA).