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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Traffic. 2017 Apr 4;18(5):315–329. doi: 10.1111/tra.12479

Figure 7. The dgk1Δ fusion effect is in parallel to the function of Yck3.

Figure 7

(A–B) Immunoblotting and quantitation of Yck3 content on vacuoles from wild type and dgk1Δ cells. (C) Immunoblotting of Mon1 release from vacuoles during the fusion reaction. Fusion reactions were incubated for 0, 30, or 60 min after which membranes (bound) and supernatants (unbound) were separated by centrifugation (13,000 g, 15 min, 4°C). (D) Quantitation of bound Mon1 at the indicated times. (E) Fusion reactions containing were treated with indicated concentrations of recombinant His6-Yck3. (F) Fusion of wild type, dgk1Δ, yck3Δ and dgk1Δ/yck3Δ vacuoles. Fusion reactions were incubated for 90 min at 27°C. Error bars represent S.E.M. (n=3).