Fig. 1. Experimental procedure for studying surface glycoprotein dynamics and measuring their half-lives. All six flasks of cells were labelled in DMEM containing 100 μM GalNAz for 24 h, then cell surface glycoproteins were tagged with click chemistry under physiological conditions. After tagging, media were switched to the normal chase media, and each flask of cells was harvested at a different time point (0, 2, 4, 6, 8 or 10 h). Enriched glycopeptides were labelled with TMT reagents for quantification.
